Advancing high-throughput gene synthesis technology

Jingdong Tian; Kuosheng Ma; Ishtiaq Saaem

doi:10.1039/B822268C

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Molecular BioSystems

Issue 7, 2009

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Advancing high-throughput gene synthesis technology

Jingdong Tian , Kuosheng Ma and Ishtiaq Saaem

Mol. BioSyst., 2009, 5, 714-722

DOI: 10.1039/B822268C
Received 10 Dec 2008, Accepted 11 Feb 2009
First published on the web 06 Apr 2009
This article is part of the collection: Molecular BioSystems Review Articles in the “-omics sciences”

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Abstract
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The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potentially lead to breakthroughs in the development of accurate, low-cost and high-throughput gene synthesis technology. The capability of generating unlimited supplies of DNA molecules of any sequence or size will transform biomedical research in the near future.

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