Jump to main content
Jump to site search

Issue 24, 2009
Previous Article Next Article

Rapid multicomponent optical protein patterning

Author affiliations

Abstract

Cells sense spatial distributions of molecules which trigger signal transduction pathways that induce the cell to migrate or extend by remodelling the cytoskeleton. However, the influence of local and small variations of extracellular protein concentration on chemotaxis is not fully understood, due in part to the lack of simple and precise methods to pattern proteinsin vitro. We recently developed a new technology to fabricate such patterns which relies on photobleaching fluorophores to adsorb proteins on a cell culture substrate: laser-assisted protein adsorption by photobleaching (LAPAP). Here we report several key improvements to LAPAP: we created arbitrary patterns made of several different proteins simultaneously, we reduced the fabrication time more than one order of magnitude and we used secondary antibodies to significantly enlarge the spectrum of proteins that can be employed. As a result, multicomponent protein gradients can be produced using reagents that are typically available in life science research laboratories on a standard inverted microscope equipped with a camera port.

Graphical abstract: Rapid multicomponent optical protein patterning

Back to tab navigation

Publication details

The article was received on 18 Jun 2009, accepted on 15 Sep 2009 and first published on 15 Oct 2009


Article type: Paper
DOI: 10.1039/B911967A
Citation: Lab Chip, 2009,9, 3580-3585
  •   Request permissions

    Rapid multicomponent optical protein patterning

    J. M. Bélisle, D. Kunik and S. Costantino, Lab Chip, 2009, 9, 3580
    DOI: 10.1039/B911967A

Search articles by author

Spotlight

Advertisements