An integrated microfluidic device for large-scale in situ click chemistry screening

Yanju Wang; Wei-Yu Lin; Kan Liu; Rachel J. Lin; Matthias Selke; Hartmuth C. Kolb; Nangang Zhang; Xing-Zhong Zhao; Michael E. Phelps; Clifton K. F. Shen; Kym F. Faull; Hsian-Rong Tseng

doi:10.1039/B907430A

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Lab on a Chip

Issue 16, 2009

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An integrated microfluidic device for large-scale in situ click chemistry screening

Yanju Wang , Wei-Yu Lin , Kan Liu , Rachel J. Lin , Matthias Selke , Hartmuth C. Kolb , Nangang Zhang , Xing-Zhong Zhao , Michael E. Phelps , Clifton K. F. Shen , Kym F. Faull and Hsian-Rong Tseng

Lab Chip, 2009, 9, 2281-2285

DOI: 10.1039/B907430A
Received 14 Apr 2009, Accepted 11 Jun 2009
First published on the web 17 Jun 2009

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An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.

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