Jump to main content
Jump to site search

Issue 14, 2009
Previous Article Next Article

Inkjet formation of unilamellar lipid vesicles for cell-like encapsulation

Author affiliations

Abstract

Encapsulation of macromolecules within lipid vesicles has the potential to drive biological discovery and enable development of novel, cell-like therapeutics and sensors. However, rapid and reliable production of large numbers of unilamellar vesicles loaded with unrestricted and precisely-controlled contents requires new technologies that overcome size, uniformity, and throughput limitations of existing approaches. Here we present a high-throughput microfluidic method for vesicle formation and encapsulation using an inkjet printer at rates up to 200 Hz. We show how multiple high-frequency pulses of the inkjet's piezoelectric actuator create a microfluidic jet that deforms a bilayer lipid membrane, controlling formation of individual vesicles. Variations in pulse number, pulse voltage, and solution viscosity are used to control the vesicle size. As a first step toward cell-like reconstitution using this method, we encapsulate the cytoskeletal protein actin and use co-encapsulated microspheres to track its polymerization into a densely entangled cytoskeletal network upon vesicle formation.

Graphical abstract: Inkjet formation of unilamellar lipid vesicles for cell-like encapsulation

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 11 Mar 2009, accepted on 27 May 2009 and first published on 08 Jun 2009


Article type: Paper
DOI: 10.1039/B904984C
Citation: Lab Chip, 2009,9, 2003-2009
  •   Request permissions

    Inkjet formation of unilamellar lipid vesicles for cell-like encapsulation

    J. C. Stachowiak, D. L. Richmond, T. H. Li, F. Brochard-Wyart and D. A. Fletcher, Lab Chip, 2009, 9, 2003
    DOI: 10.1039/B904984C

Search articles by author