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Issue 16, 2009
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Simultaneous sorting of multiple bacterial targets using integrated Dielectrophoretic–Magnetic Activated Cell Sorter

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Abstract

The ability to rapidly and accurately sort multiple types of biological targets—such as molecules, viruses, bacteria or mammalian cells—from complex sample mixtures is an essential component for a wide range of diagnostic and therapeutic strategies. However, most current selection methods for cell separation are either limited with regard to throughput, as is the case for Fluorescence Assisted Cell Sorting (FACS), or else only allow binary separation of targets that have been labeled via a single parameter, such as Magnetic Activated Cell Sorting (MACS). We report here the integrated Dielectrophoretic–Magnetic Activated Cell Sorter (iDMACS), an integrated platform that combines two different force fields in a single microfluidic device for highly efficient multi-target separation. We describe the underlying physics and design of the iDMACS device and demonstrate ∼900-fold enrichment of multiple bacterial target cell types with over 95% purity after a single round of separation.

Graphical abstract: Simultaneous sorting of multiple bacterial targets using integrated Dielectrophoretic–Magnetic Activated Cell Sorter

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Publication details

The article was received on 25 Feb 2009, accepted on 01 May 2009 and first published on 14 May 2009


Article type: Paper
DOI: 10.1039/B903950C
Citation: Lab Chip, 2009,9, 2313-2318
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    Simultaneous sorting of multiple bacterial targets using integrated Dielectrophoretic–Magnetic Activated Cell Sorter

    U. Kim and H. T. Soh, Lab Chip, 2009, 9, 2313
    DOI: 10.1039/B903950C

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