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Issue 12, 2009
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Selective and tunable gradient device for cell culture and chemotaxis study

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Abstract

This article describes a microfluidic device for cell culture and chemotaxis studies under various temporal and spatial concentration gradients of the medium or chemoattractant. Vertical membranes formed using in situ fabrication are employed to avoid fluid flow inside the cell observation chamber. Thus, the medium and chemoattractants are primarily provided by diffusion, maintaining cell–cell communicationvia secreted factors. Neutrophils were used to demonstrate the capability of the device for chemotaxis research. Experiments exhibited successful migration up a concentration gradient of interleukin 8.

Graphical abstract: Selective and tunable gradient device for cell culture and chemotaxis study

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Publication details

The article was received on 27 Jan 2009, accepted on 16 Mar 2009 and first published on 26 Mar 2009


Article type: Technical Note
DOI: 10.1039/B901613A
Citation: Lab Chip, 2009,9, 1797-1800
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    Selective and tunable gradient device for cell culture and chemotaxis study

    D. Kim, M. A. Lokuta, A. Huttenlocher and D. J. Beebe, Lab Chip, 2009, 9, 1797
    DOI: 10.1039/B901613A

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