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Issue 16, 2009
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Patch clamping on plane glass—fabrication of hourglass aperture and high-yield ion channel recording

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Abstract

Planar patch-clamp has revolutionized ion-channel measurement by eliminating laborious manipulation from the traditional micropipette approach and enabling high throughput. However, low yield in gigaseal formation and/or relatively high cost due to microfabricated processes are two main drawbacks. This paper presents patch clamping on glass substrate—an economical solution without sacrificing gigaseal yield rate. Two-stage CO2 laser drilling methodology was used to generate an hourglass, funnel-like aperture of a specified diameter with smooth and debris-free surfaces on 150 µm borosilicate cover glass. For 1–3 µm apertures as patch-clamp chips, seal resistance was tested on human embryonic kidney, Chinese hamster ovary, and Jurkat T lymphoma cells with a gigaseal success rate of 62.5%, 43.6% and 66.7% respectively. Results also demonstrated both whole-cell and single channel recording on endogenously expressed ion channels to confirm the capability of different patch configurations.

Graphical abstract: Patch clamping on plane glass—fabrication of hourglass aperture and high-yield ion channel recording

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Publication details

The article was received on 16 Jan 2009, accepted on 09 Apr 2009 and first published on 14 May 2009


Article type: Paper
DOI: 10.1039/B901025D
Citation: Lab Chip, 2009,9, 2370-2380
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    Patch clamping on plane glass—fabrication of hourglass aperture and high-yield ion channel recording

    C. Chen, T. Tu, C. Chen, D. Jong and A. M. Wo, Lab Chip, 2009, 9, 2370
    DOI: 10.1039/B901025D

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