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Issue 14, 2009
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A microfluidic 3D hepatocyte chip for drug toxicity testing

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Abstract

We have developed a microfluidic 3D hepatocyte chip (3D HepaTox Chip) for in vitro drug toxicity testing to predict in vivo drug hepatotoxicity. The 3D HepaTox Chip is based on multiplexed microfluidic channels where a 3D microenvironment is engineered in each channel to maintain the hepatocytes' synthetic and metabolic functions. The multiplexed channels allow for simultaneous administration of multiple drug doses to functional primary hepatocytes while an incorporated concentration gradient generator enables the in vitro dose-dependent drug responses to predict in vivo hepatotoxicity. The IC50 values of 5 model drugs derived from the dose-dependent on-chip testing correlate well with the reported in vivo LD50 values. The 3D HepaTox Chip can be integrated with on-chip sensors and actuators as the next generation cell-based on-chip drug testing platform.

Graphical abstract: A microfluidic 3D hepatocyte chip for drug toxicity testing

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Publication details

The article was received on 15 Jan 2009, accepted on 01 Apr 2009 and first published on 20 Apr 2009


Article type: Paper
DOI: 10.1039/B900912D
Citation: Lab Chip, 2009,9, 2026-2035
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    A microfluidic 3D hepatocyte chip for drug toxicity testing

    Y. Toh, T. C. Lim, D. Tai, G. Xiao, D. van Noort and H. Yu, Lab Chip, 2009, 9, 2026
    DOI: 10.1039/B900912D

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