Characterizing dispersion in microfluidic channels

Subhra Datta; Sandip Ghosal

doi:10.1039/B822948C

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Lab on a Chip

Issue 17, 2009

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Characterizing dispersion in microfluidic channels

Subhra Datta and Sandip Ghosal

Lab Chip, 2009, 9, 2537-2550

DOI: 10.1039/B822948C
Received 19 Dec 2008, Accepted 27 May 2009
First published on the web 12 Aug 2009
This article is part of the collection: Fundamental Principles and Techniques in Microfluidics

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Dispersion or spreading of analyte bands is a barrier to achieving high resolution in microfluidic separations. The role of dispersion in separations is reviewed with emphasis on metrics, sources and common principles of analysis. Three sources of dispersion (a) inhomogeneous flow fields, (b) solute wall interactions and (c) force fields normal to channel walls are studied in detail. Microfluidic and nanofluidic applications to capillary electrophoresis, chromatography and field-flow fractionation, that are subject to one or more of these three physical processes under standard, unintentional or novel operating conditions, are discussed.

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