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Issue 14, 2009
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DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array

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Abstract

A microdevice is developed for DNA-barcode directed capture of single cells on an array of pH-sensitive microelectrodes for metabolic analysis. Cells are modified with membrane-bound single-stranded DNA, and specific single-cell capture is directed by the complementary strand bound in the sensor area of the iridium oxide pH microelectrodes within a microfluidic channel. This bifunctional microelectrode array is demonstrated for the pH monitoring and differentiation of primary T cells and Jurkat T lymphoma cells. Single Jurkat cells exhibited an extracellular acidification rate of 11 milli-pH min−1, while primary T cells exhibited only 2 milli-pH min−1. This system can be used to capture non-adherent cells specifically and to discriminate between visually similar healthy and cancerous cells in a heterogeneous ensemble based on their altered metabolic properties.

Graphical abstract: DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array

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Publication details

The article was received on 03 Dec 2008, accepted on 26 Mar 2009 and first published on 15 Apr 2009


Article type: Paper
DOI: 10.1039/B821690H
Citation: Lab Chip, 2009,9, 2010-2015
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    DNA-barcode directed capture and electrochemical metabolic analysis of single mammalian cells on a microelectrode array

    E. S. Douglas, S. C. Hsiao, H. Onoe, C. R. Bertozzi, M. B. Francis and R. A. Mathies, Lab Chip, 2009, 9, 2010
    DOI: 10.1039/B821690H

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