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Issue 12, 2009
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Parallel single-cell light-induced electroporation and dielectrophoretic manipulation

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Abstract

Electroporation is a common technique for the introduction of exogenous molecules across the, otherwise, impermeant cell membrane. Conventional techniques are limited by either low throughput or limited selectivity. Here we present a novel technique whereby we use patterned light to create virtual electrodes which can induce the parallel electroporation of single cells. This technique seamlessly integrates with optoelectronic tweezers to provide a single cell manipulation platform as well. We present evidence of parallel, single cell electroporation using this method through use of fluorescent dyes and dielectrophoretic responses. Additionally, through the use of integrated microfluidic channels, we show that cells remain viable following treatment in the device. Finally, we determine the optimal field dosage to inject propidium iodide into a HeLa cell and maintain cellular viability.

Graphical abstract: Parallel single-cell light-induced electroporation and dielectrophoretic manipulation

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Publication details

The article was received on 03 Dec 2008, accepted on 20 Feb 2009, published on 13 Mar 2009 and first published online on 13 Mar 2009


Article type: Paper
DOI: 10.1039/B821678A
Citation: Lab Chip, 2009,9, 1714-1720
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    Parallel single-cell light-induced electroporation and dielectrophoretic manipulation

    J. K. Valley, S. Neale, H. Hsu, A. T. Ohta, A. Jamshidi and M. C. Wu, Lab Chip, 2009, 9, 1714
    DOI: 10.1039/B821678A

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