Berkeley Sensor and Actuator Center, Department of Electrical Engineering and Computer Science, University of California Berkeley, 497 Cory Hall, Berkeley, USA
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Lab Chip, 2009,9, 1714-1720
03 Dec 2008,
20 Feb 2009
First published online
13 Mar 2009
Electroporation is a common technique for the introduction of exogenous molecules across the, otherwise, impermeant cell membrane. Conventional techniques are limited by either low throughput or limited selectivity. Here we present a novel technique whereby we use patterned light to create virtual electrodes which can induce the parallel electroporation of single cells. This technique seamlessly integrates with optoelectronic tweezers to provide a single cell manipulation platform as well. We present evidence of parallel, single cellelectroporation using this method through use of fluorescent dyes and dielectrophoretic responses. Additionally, through the use of integrated microfluidic channels, we show that cells remain viable following treatment in the device. Finally, we determine the optimal field dosage to inject propidium iodide into a HeLa cell and maintain cellular viability.
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