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Issue 14, 2009
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Three-dimensional micro-electrode array for recording dissociated neuronal cultures

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Abstract

This work demonstrates the design, fabrication, packaging, characterization, and functionality of an electrically and fluidically active three-dimensional micro-electrode array (3D MEA) for use with neuronal cell cultures. The successful function of the device implies that this basic concept—construction of a 3D array with a layered approach—can be utilized as the basis for a new family of neural electrode arrays. The 3D MEA prototype consists of a stack of individually patterned thin films that form a cell chamber conducive to maintaining and recording the electrical activity of a long-term three-dimensional network of rat cortical neurons. Silicon electrode layers contain a polymer grid for neural branching, growth, and network formation. Along the walls of these electrode layers lie exposed gold electrodes which permit recording and stimulation of the neuronal electrical activity. Silicone elastomer micro-fluidic layers provide a means for loading dissociated neurons into the structure and serve as the artificial vasculature for nutrient supply and aeration. The fluidic layers also serve as insulation for the micro-electrodes. Cells have been shown to survive in the 3D MEA for up to 28 days, with spontaneous and evoked electrical recordings performed in that time. The micro-fluidic capability was demonstrated by flowing in the drug tetrotodoxin to influence the activity of the culture.

Graphical abstract: Three-dimensional micro-electrode array for recording dissociated neuronal cultures

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Publication details

The article was received on 19 Nov 2008, accepted on 25 Mar 2009 and first published on 08 Apr 2009


Article type: Paper
DOI: 10.1039/B820596E
Citation: Lab Chip, 2009,9, 2036-2042
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    Three-dimensional micro-electrode array for recording dissociated neuronal cultures

    K. Musick, D. Khatami and B. C. Wheeler, Lab Chip, 2009, 9, 2036
    DOI: 10.1039/B820596E

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