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Issue 10, 2009
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Automated microfluidic chromatin immunoprecipitation from 2,000 cells

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Abstract

Chromatin immunoprecipitation (ChIP) is a powerful assay used to probe DNA–protein interactions. Traditional methods of implementing this assay are lengthy, cumbersome and require a large number of cells, making it difficult to study rare cell types such as certain cancer and stem cells. We have designed a microfluidic device to perform sensitive ChIP analysis on low cell numbers in a rapid, automated fashion while preserving the specificity of the assay. Comparing ChIP results for two modified histone protein targets, we showed our automated microfluidic ChIP (AutoChIP) from 2,000 cells to be comparable to that of conventional ChIP methods using 50,000–500,000 cells. This technology may provide a solution to the need for a high sensitivity, rapid, and automated ChIP assay, and in doing so facilitate the use of ChIP for many interesting and valuable applications.

Graphical abstract: Automated microfluidic chromatin immunoprecipitation from 2,000 cells

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Publication details

The article was received on 05 Nov 2008, accepted on 04 Feb 2009 and first published on 27 Feb 2009


Article type: Paper
DOI: 10.1039/B819648F
Citation: Lab Chip, 2009,9, 1365-1370
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    Automated microfluidic chromatin immunoprecipitation from 2,000 cells

    A. R. Wu, J. B. Hiatt, R. Lu, J. L. Attema, N. A. Lobo, I. L. Weissman, M. F. Clarke and S. R. Quake, Lab Chip, 2009, 9, 1365
    DOI: 10.1039/B819648F

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