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Issue 10, 2009
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Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis

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Abstract

Microfluidic devices were designed to perform on micromoles of biological macromolecules and viruses the search and the optimization of crystallization conditions by counter-diffusion, as well as the on-chip analysis of crystals by X-ray diffraction. Chips composed of microchannels were fabricated in poly-dimethylsiloxane (PDMS), poly-methyl-methacrylate (PMMA) and cyclo-olefin-copolymer (COC) by three distinct methods, namely replica casting, laser ablation and hot embossing. The geometry of the channels was chosen to ensure that crystallization occurs in a convection-free environment. The transparency of the materials is compatible with crystal growth monitoring by optical microscopy. The quality of the protein 3D structures derived from on-chip crystal analysis by X-ray diffraction using a synchrotron radiation was used to identify the most appropriate polymers. Altogether the results demonstrate that for a novel biomolecule, all steps from the initial search of crystallization conditions to X-ray diffraction data collection for 3D structure determination can be performed in a single chip.

Graphical abstract: Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis

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Publication details

The article was received on 03 Nov 2008, accepted on 30 Jan 2009 and first published on 02 Mar 2009


Article type: Paper
DOI: 10.1039/B819362B
Citation: Lab Chip, 2009,9, 1412-1421
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    Microfluidic chips for the crystallization of biomacromolecules by counter-diffusion and on-chip crystal X-ray analysis

    K. Dhouib, C. Khan Malek, W. Pfleging, B. Gauthier-Manuel, R. Duffait, G. Thuillier, R. Ferrigno, L. Jacquamet, J. Ohana, J. Ferrer, A. Théobald-Dietrich, R. Giegé, B. Lorber and C. Sauter, Lab Chip, 2009, 9, 1412
    DOI: 10.1039/B819362B

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