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Issue 7, 2009
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Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles

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Abstract

The application of biomolecular active transport systems offers a potential route for downscaling multiple analyte assays for lab-on-a-chip applications. Recently, the capture and transport of a wide range of target analytes including proteins, virus particles, and DNA have been demonstrated using kinesin-driven molecular shuttles. The molecular shuttles consisted of microtubule (MT) filaments that were functionalized with either analyte-selective antibodies or complementary DNA, thus facilitating selective target capture and transport. In the present work, we have applied this microfluidic platform for the simultaneous detection of multiple target protein analytes. Multiplexing of molecular shuttles was achieved by immobilizing biotinylated antibodies against interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α) on biotinylated MTs using a streptavidin bridge. Nanocrystal quantum dots of different sizes and spectral emissions were functionalized with IL-2 and TNF-α antibodies to facilitate multiplexed detection. In this paper we discuss the results of selectivity and motility in single and multiplexed assays.

Graphical abstract: Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles

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Publication details

The article was received on 19 Sep 2008, accepted on 28 Nov 2008 and first published on 05 Jan 2009


Article type: Technical Note
DOI: 10.1039/B816444D
Citation: Lab Chip, 2009,9, 1005-1010
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    Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles

    L. Rios and G. D. Bachand, Lab Chip, 2009, 9, 1005
    DOI: 10.1039/B816444D

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