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Issue 7, 2009
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Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip

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Abstract

We use novel super-resolution bright-field optical microscopy to observe the filopodium activities of human lung cancer cells in a multi-gradient cell culture chip. Temporal variations of the filopodium numbers are measured without fluorescent labelling. By carefully designing the fluidic field inside the culture chip, we establish stable concentration gradients of the injected reagents. The reagents are injected via a separated central inlet, and the concentration gradients are different at different positions in the chip. The same chip can be used for both control and treated experiments. Using epidermal growth factor as the treatment, we verify that the protrusions of filopodia indicate the direction of concentration gradients experienced by a living cancer cell; while the treatment of bovine serum albumin shows no specific effect on the growth of filopodia. The combination of label-free, high-resolution optical microscopy and a micro cell culture chip establishes a convenient and versatile platform for dynamical cancer-cell analyses.

Graphical abstract: Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip

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Publication details

The article was received on 19 Aug 2008, accepted on 12 Dec 2008 and first published on 15 Jan 2009


Article type: Paper
DOI: 10.1039/B814405B
Citation: Lab Chip, 2009,9, 884-890
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    Label-free quantification of asymmetric cancer-cell filopodium activities in a multi-gradient chip

    T. Hsu, M. Yen, W. Liao, J. Cheng and C. Lee, Lab Chip, 2009, 9, 884
    DOI: 10.1039/B814405B

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