Computer-enhanced high-throughput genetic screens of C. elegans in a microfluidic system

Matthew M. Crane; Kwanghun Chung; Hang Lu

doi:10.1039/B813730G

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Lab on a Chip

Issue 1, 2009

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Computer-enhanced high-throughput genetic screens of C. elegans in a microfluidic system

Matthew M. Crane , Kwanghun Chung and Hang Lu

Lab Chip, 2009, 9, 38-40

DOI: 10.1039/B813730G
Received 07 Aug 2008, Accepted 17 Oct 2008
First published on the web 27 Oct 2008

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Abstract
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Most visual screens based on fluorescent markers are currently limited in throughput and accuracy. Here we present the first genetic screen of a mutagenized population of C. elegans in a microfluidic device. Animal handling streamlined by a microfluidic device and intuitive control software enabled the identification of novel mutants and a large screening speed.

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Details regarding the device fabrication and operation
PDF (277K)

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Computer-enhanced high-throughput genetic screens of C. elegans in a microfluidic system

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