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Issue 5, 2009
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Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

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Abstract

We describe the design, fabrication and use of a single-layered poly(dimethylsiloxane) microfluidic structure for the entrapment and release of microdroplets in an array format controlled entirely by liquid flow. Aqueous picoliter droplets are trapped en masse and optically monitored for extended periods of time. Such an array-based approach is used to characterize droplet shrinkage, aggregation of encapsulated E. colicells and enzymatic reactions. We also demonstrate that trapped droplets may be recovered from the microfluidic array for further processing.

Graphical abstract: Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

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Publication details

The article was received on 07 Aug 2008, accepted on 14 Oct 2008 and first published on 27 Nov 2008


Article type: Paper
DOI: 10.1039/B813709A
Citation: Lab Chip, 2009,9, 692-698
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    Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays

    A. Huebner, D. Bratton, G. Whyte, M. Yang, A. J. deMello, C. Abell and F. Hollfelder, Lab Chip, 2009, 9, 692
    DOI: 10.1039/B813709A

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