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Issue 3, 2009
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Novel MEA platform with PDMS microtunnels enables the detection of action potential propagation from isolated axons in culture

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Abstract

This study investigated a novel multi-electrode-array (MEA) design capable of long-term and highly selective recordings of axonal signals using PDMS microtunnels. We successfully grew neurons in culture so that only axons extended through narrow (10 µm wide by 3 µm high) and long (750 µm) microtunnels under which multiple electrodes were integrated. This permitted the recording of relatively large (up to 200 µV) electrical signals, including the propagation speed and direction of these travelling action potentials. To further demonstrate the operation of the device as a diagnostic tool for drug screening assays, the drug mepivacaine was applied in washout experiments. Here, we identified significant changes in mean spiking rate and conduction velocity.

Graphical abstract: Novel MEA platform with PDMS microtunnels enables the detection of action potential propagation from isolated axons in culture

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Publication details

The article was received on 23 Apr 2008, accepted on 08 Oct 2008 and first published on 18 Nov 2008


Article type: Paper
DOI: 10.1039/B806689B
Citation: Lab Chip, 2009,9, 404-410
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    Novel MEA platform with PDMS microtunnels enables the detection of action potential propagation from isolated axons in culture

    B. J. Dworak and B. C. Wheeler, Lab Chip, 2009, 9, 404
    DOI: 10.1039/B806689B

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