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Issue 12, 2009
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EWOD-driven droplet microfluidic device integrated with optoelectronic tweezers as an automated platform for cellular isolation and analysis

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Abstract

We report the integration of two technologies: droplet microfluidics using electrowetting-on-dielectric (EWOD) and individual particle manipulation using optoelectronic tweezers (OET)—in one microfluidic device. The integrated device successfully demonstrates a sequence involving both EWOD and OET operations. We encountered various challenges during integration of the two different technologies and present how they are addressed. To show the applicability of the device in cellular biology, live HeLa cells are used in the experiments. The unique advantages of EWOD and OET make their integration a significant step towards a powerful tool for many applications, such as single cell studies involving multiplexed environmental stimuli.

Graphical abstract: EWOD-driven droplet microfluidic device integrated with optoelectronic tweezers as an automated platform for cellular isolation and analysis

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Publication details

The article was received on 02 Dec 2008, accepted on 04 Mar 2009, published on 16 Mar 2009 and first published online on 16 Mar 2009


Article type: Paper
DOI: 10.1039/B821508A
Citation: Lab Chip, 2009,9, 1732-1739
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    EWOD-driven droplet microfluidic device integrated with optoelectronic tweezers as an automated platform for cellular isolation and analysis

    G. J. Shah, A. T. Ohta, E. P.-Y. Chiou, M. C. Wu and C. “. Kim, Lab Chip, 2009, 9, 1732
    DOI: 10.1039/B821508A

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