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Issue 4, 2009
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Protein self-interaction chromatography on a microchip

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Abstract

This paper presents the development of a novel miniaturized experimental procedure for the measurement of proteinprotein interactions through Self-Interaction Chromatography (SIC) on a microchip, without the use of chromatographic resins. SIC was recently demonstrated to be a relatively easy method to obtain quantitative thermodynamic information about proteinprotein interactions, like the osmotic second virial coefficient B22, which relates to protein phase behavior including protein crystallization. This successful miniaturization to microchip level of a measurement device for protein self-interaction data is a first key step to a complete microfluidic screening platform for the rational design of protein crystallizations, using substantially less expensive protein and experimentation time.

Graphical abstract: Protein self-interaction chromatography on a microchip

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Publication details

The article was received on 03 Jul 2008, accepted on 30 Oct 2008 and first published on 21 Nov 2008


Article type: Paper
DOI: 10.1039/B810741F
Citation: Lab Chip, 2009,9, 600-605
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    Protein self-interaction chromatography on a microchip

    K. Deshpande, T. Ahamed, L. A. M. van der Wielen, J. H. T. Horst, P. J. Jansens and M. Ottens, Lab Chip, 2009, 9, 600
    DOI: 10.1039/B810741F

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