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Issue 2, 2009
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Single cell detection using a glass-based optofluidic device fabricated by femtosecond laser pulses

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Abstract

We demonstrate the fabrication of integrated three-dimensional microchannel and optical waveguide structures inside fused silica for the interrogation and processing of single cells. The microchannels are fabricated by scanning femtosecond laser pulses (523 nm) and subsequent selective wet etching process. Optical waveguides are additionally integrated with the fabricated microchannels by scanning the laser pulse train inside the glass specimen. Single red blood cells (RBC) in diluted human blood inside of the manufactured microchannel were detected by two optical schemes. The first involved sensing the intensity change of waveguide-delivered He–Ne laser light (632.8 nm) induced by the refractive index difference of a cell flowing in the channel. The other approach was viadetection of fluorescence emission from dyed RBC excited by Ar laser light (488 nm) delivered by the optical waveguide. The proposed device was tested to detect 23 fluorescent particles per second by increasing the flow rate up to 0.5 μl min−1. The optical cell detection experiments support potential implementation of a new generation of glass-based optofluidic biochip devices in various single cell treatment processes including laser based cell processing and sensing.

Graphical abstract: Single cell detection using a glass-based optofluidic device fabricated by femtosecond laser pulses

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Publication details

The article was received on 19 May 2008, accepted on 18 Aug 2008 and first published on 23 Oct 2008


Article type: Paper
DOI: 10.1039/B808366E
Citation: Lab Chip, 2009,9, 311-318
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    Single cell detection using a glass-based optofluidic device fabricated by femtosecond laser pulses

    M. Kim, D. J. Hwang, H. Jeon, K. Hiromatsu and C. P. Grigoropoulos, Lab Chip, 2009, 9, 311
    DOI: 10.1039/B808366E

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