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Issue 1, 2009
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Photobleaching absorbed Rhodamine B to improve temperature measurements in PDMS microchannels

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Abstract

Rhodamine B based fluorescence thermometry is commonly used in microfluidics to measure fluid temperatures in microchannels. Notable absorption of Rhodamine B into PDMS channel walls, however, causes difficulties in obtaining accurate temperature measurements due to a steady increase in the overall fluorescent signal. A simple and effective technique is reported that removes the fluorescent signal from absorbed Rhodamine B dye by means of photobleaching with a high intensity light source before taking images for thermometry analysis. The temperature field at the convergence of hot and cold streams in a Y-channel fabricated in PDMS/glass microfluidic chip is studied to demonstrate the execution of the photobleaching technique.

Graphical abstract: Photobleaching absorbed Rhodamine B to improve temperature measurements in PDMS microchannels

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Publication details

The article was received on 27 Mar 2008, accepted on 19 Aug 2008 and first published on 20 Oct 2008


Article type: Technical Note
DOI: 10.1039/B805172K
Citation: Lab Chip, 2009,9, 171-174
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    Photobleaching absorbed Rhodamine B to improve temperature measurements in PDMS microchannels

    T. Glawdel, Z. Almutairi, S. Wang and C. Ren, Lab Chip, 2009, 9, 171
    DOI: 10.1039/B805172K

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