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Issue 35, 2009
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Glycosaminoglycan-functionalized gold nanorods: interactions with cardiac cells and type I collagen

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Abstract

The sulfated glycosaminoglycans (sGAG) heparin and chondroitin sulfate (CS) were immobilized on the surfaces of gold nanorods as part of a polyelectrolyte multilayer. The effects of these nanomaterials on the self-assembly of type I collagen were examined by turbidity assays and microscopy, and desorption of sGAG from nanomaterial-collagen composites was quantified biochemically. The interactions of sGAG-coated nanorods with cardiac cells were also explored through a collagen gel contraction assay and confocal microscopy. In contrast to soluble forms of sGAG, sGAG-coated nanorods consistently accelerated collagen fibrillogenesis. Soluble heparin, and heparin- and CS-coated nanorods inhibited cell-mediated contraction of collagen gels, whereas soluble CS did not. Both heparin and CS-coated nanorods were detected in the peri- and/or intra-cellular compartments of the cells, but there was no evidence of cytotoxicity over 72 h of culture. These results indicate that biological polyanions, such as sGAG, may be useful in the modification of nanoparticle surface chemistry for biological and/or therapeutic applications.

Graphical abstract: Glycosaminoglycan-functionalized gold nanorods: interactions with cardiac cells and type I collagen

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Publication details

The article was received on 10 Feb 2009, accepted on 23 Apr 2009 and first published on 27 May 2009


Article type: Paper
DOI: 10.1039/B902760B
Citation: J. Mater. Chem., 2009,19, 6332-6340
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    Glycosaminoglycan-functionalized gold nanorods: interactions with cardiac cells and type I collagen

    C. G. Wilson, P. N. Sisco, E. C. Goldsmith and C. J. Murphy, J. Mater. Chem., 2009, 19, 6332
    DOI: 10.1039/B902760B

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