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Issue 1, 2009
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LC-ICP-MS and LC-ESI-(MS)n identification of Se-methylselenocysteine and selenomethionine as metabolites of methylseleninic acid in rat hepatocytes

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Abstract

The metabolism of methylseleninic acid in isolated rat hepatocytes was investigated. Selenium containing metabolites excreted from the cells were detected in the supernatant of the incubation sample by LC-ICP-MS. After pre-treatment of the supernatant by preparative chromatography and pre-concentration by lyophilisation, a major metabolite was identified by molecular mass spectrometry as Se-methylselenocysteine by LC-ESI-MS, MS2 and MS3 and a minor metabolite was identified as selenomethionine by LC-ESI-MS2 and MS3 and LC-ESI-MS2(SRM). This is the first time these metabolites have been identified in hepatocytes. Complementary data from ion trap and triple quadrupole MS instruments provided solid proof of metabolite identities. A time course study showed that S-(methylseleno)cysteine and S-(methylseleno)glutathione were intermediates in the formation of the major metabolite. It is questioned if methylseleninic acid is a relevant model compound for methylated Se-amino acids in vitro.

Graphical abstract: LC-ICP-MS and LC-ESI-(MS)n identification of Se-methylselenocysteine and selenomethionine as metabolites of methylseleninic acid in rat hepatocytes

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Publication details

The article was received on 07 May 2008, accepted on 15 Sep 2008 and first published on 16 Oct 2008


Article type: Paper
DOI: 10.1039/B807805J
Citation: J. Anal. At. Spectrom., 2009,24, 69-75
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    LC-ICP-MS and LC-ESI-(MS)n identification of Se-methylselenocysteine and selenomethionine as metabolites of methylseleninic acid in rat hepatocytes

    C. Gabel-Jensen, J. Odgaard, C. Skonberg, L. Badolo and B. Gammelgaard, J. Anal. At. Spectrom., 2009, 24, 69
    DOI: 10.1039/B807805J

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