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Issue 5, 2009
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Monitoring of redox state in a dechlorinating culture with immobilized redox indicators

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Abstract

During the dechlorination of tetrachloroethene (PCE) to ethene (ETH) in a microcosm bottle containing an enriched chlororespiring culture, redox conditions were spectrophotometrically monitored with the redox indicators thionine (THI) and cresyl violet (CV) immobilized on transparent films. Measurements were taken with a unique flow redox monitoring system based on circulating solution from the bottle through a specially constructed flow cell and returning it to the bottle. An enclosure for a peristaltic pump was constructed to minimize contamination of the solution with atmospheric O2. The redox monitoring instrumentation was shown to be an excellent tool to evaluate the ability of an enclosure for anoxic samples to prevent diffusion of very small amounts of oxygen from the atmosphere into the sample. With careful design of the apparatus, the O2 permeation rate into a solution of THI in a microcosm bottle during redox monitoring was reduced to less than 40 nmol h−1. When monitoring a dechlorinating culture, dechlorination was not observed until immobilized THI was completely reduced and vinyl chloride was not produced until partial reduction of CV.

Graphical abstract: Monitoring of redox state in a dechlorinating culture with immobilized redox indicators

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Publication details

The article was received on 21 Oct 2008, accepted on 03 Feb 2009 and first published on 27 Feb 2009


Article type: Paper
DOI: 10.1039/B818487A
Citation: J. Environ. Monit., 2009,11, 1028-1036
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    Monitoring of redox state in a dechlorinating culture with immobilized redox indicators

    P. Ruiz-Haas and J. Ingle, Jr., J. Environ. Monit., 2009, 11, 1028
    DOI: 10.1039/B818487A

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