Genetically encoded biosensors based on engineered fluorescent proteins

Wolf B. Frommer; Michael W. Davidson; Robert E. Campbell

doi:10.1039/B907749A

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Chemical Society Reviews

Issue 10, 2009

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Genetically encoded biosensors based on engineered fluorescent proteins

Wolf B. Frommer , Michael W. Davidson and Robert E. Campbell

Chem. Soc. Rev., 2009, 38, 2833-2841

DOI: 10.1039/B907749A
Received 20 Apr 2009, First published on the web 04 Aug 2009
This article is part of the collection: Green Fluorescent Protein

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Fluorescent proteins have revolutionized cell biology by allowing researchers to non-invasively peer into the inner workings of cells and organisms. While the most common applications of fluorescent proteins are to image expression, localization, and dynamics of protein chimeras, there is a growing interest in using fluorescent proteins to create biosensors for minimally invasive imaging of concentrations of ions and small molecules, the activity of enzymes, and changes in the conformation of proteins in living cells. This tutorial review provides an overview of the progress made in the development of fluorescent protein-based biosensors to date.

Abstract from Analytical Abstracts 2010

Supplementary Info

A list of published biosensor constructs
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Chemical Society Reviews

Genetically encoded biosensors based on engineered fluorescent proteins

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