GFP: from jellyfish to the Nobel prize and beyond

Marc Zimmer

doi:10.1039/B904023D

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Chemical Society Reviews

Issue 10, 2009

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GFP: from jellyfish to the Nobel prize and beyond

Marc Zimmer

Chem. Soc. Rev., 2009, 38, 2823-2832

DOI: 10.1039/B904023D
First published on the web 15 Jun 2009
This article is part of the collection: Green Fluorescent Protein

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On December 10, 2008 Osamu Shimomura, Martin Chalfie and Roger Tsien were awarded the Nobel Prize in Chemistry for “the discovery and development of the green fluorescent protein, GFP”. The path taken by this jellyfish protein to become one of the most useful tools in modern science and medicine is described. Osamu Shimomura painstakingly isolated GFP from hundreds of thousands of jellyfish, characterized the chromophore and elucidated the mechanism of Aequorean bioluminescence. Martin Chalfie expressed the protein in E. coli and C. elegans, and Roger Tsien developed a palette of fluorescent proteins that could be used in a myriad of applications.

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GFP: from jellyfish to the Nobel prize and beyond

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