Jump to main content
Jump to site search

Issue 34, 2009
Previous Article Next Article

SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods

Author affiliations

Abstract

Antibody-conjugated gold nanorods (GNRs) have been used for the targeting and imaging of specific cancer markers expressed on the surface membrane of cancer cells. GNRs with various aspect ratios were fabricated, and their surface-enhanced Raman scattering enhancement effects were evaluated. To attach the GNRs selectively onto the targets in cancer cells, specific antibodies were immobilized on the surface of GNRs using the layer-by-layer deposition method. First, Raman reporter molecules, mercaptopyridine, were attached to the surface of GNRs, and their surface charge was modified using poly(sodium 4-styrene-sulfonate) to make the surface charge negative. Then, anti-rabbit IgGs were immobilized onto the surface of the GNRs by electrostatic interactions. HER2 markers, expressed on the cell surface, were treated with anti-HER2 primary antibodies. Finally, the functionalized nanoprobes, conjugated with secondary antibodies, were attached to the markers on cancer cells by antibody‚Äďantibody interactions. In the present study, MCF7 cells overexpressing breast cancer marker HER2 were used as the optical imaging targets. Our experimental results demonstrate the potential feasibility of antibody-conjugated GNRs for the highly sensitive targeting and imaging of biomarkers expressed on the surface membrane of cancer cells.

Graphical abstract: SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods

Back to tab navigation

Publication details

The article was received on 05 Mar 2009, accepted on 27 May 2009 and first published on 29 Jun 2009


Article type: Paper
DOI: 10.1039/B904592A
Citation: Phys. Chem. Chem. Phys., 2009,11, 7444-7449
  •   Request permissions

    SERS imaging of HER2-overexpressed MCF7 cells using antibody-conjugated gold nanorods

    H. Park, S. Lee, L. Chen, E. K. Lee, S. Y. Shin, Y. H. Lee, S. W. Son, C. H. Oh, J. M. Song, S. H. Kang and J. Choo, Phys. Chem. Chem. Phys., 2009, 11, 7444
    DOI: 10.1039/B904592A

Search articles by author

Spotlight

Advertisements