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Issue 24, 2009
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Using electrical and optical tweezers to facilitate studies of molecular motors

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Abstract

Dielectrophoresis was used to stretch and suspend actin filaments across a trench etched between two electrodes patterned on a glass slide. Optical tweezers were used to bring a motor protein-coated bead into close proximity to a pre-selected, suspended actin filament, facilitating the attachment of the myosin-coated bead to the filament. The clearance beneath the filament allowed the bead to move freely along and around its filamentous track, unhindered by solid surfaces. Using defocused images, the three-dimensional position of the bead was tracked as a function of time to obtain its trajectory. Experiments were carried out with myosin V and myosin X. Both motor proteins followed left-handed helical paths with the myosin X motor exhibiting a shorter pitch than the myosin V. The combined use of electrostatic and optical tweezers facilitates the preparation of motility assays with suspended tracks. Variants of this technique will enable higher complexity experiments in vitro to better understand the behavior of motors in cells.

Graphical abstract: Using electrical and optical tweezers to facilitate studies of molecular motors

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Publication details

The article was received on 05 Dec 2008, accepted on 09 Mar 2009 and first published on 16 Apr 2009


Article type: Paper
DOI: 10.1039/B821861G
Citation: Phys. Chem. Chem. Phys., 2009,11, 4834-4839
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    Using electrical and optical tweezers to facilitate studies of molecular motors

    M. E. Arsenault, Y. Sun, H. H. Bau and Y. E. Goldman, Phys. Chem. Chem. Phys., 2009, 11, 4834
    DOI: 10.1039/B821861G

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