Optimized DNA-targeting using triplex forming C5-alkynyl functionalized LNA

Sujay P. Sau; Pawan Kumar; Brooke A. Anderson; Michael E. Østergaard; Lee Deobald; Andrzej Paszczynski; Pawan K. Sharma; Patrick J. Hrdlicka

doi:10.1039/B917312A

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Chemical Communications

Issue 44, 2009

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Optimized DNA-targeting using triplex forming C5-alkynyl functionalized LNA

Sujay P. Sau , Pawan Kumar , Brooke A. Anderson , Michael E. Østergaard , Lee Deobald , Andrzej Paszczynski , Pawan K. Sharma and Patrick J. Hrdlicka

Chem. Commun., 2009, 6756-6758

DOI: 10.1039/B917312A
Received 21 Aug 2009, Accepted 17 Sep 2009
First published on the web 12 Oct 2009

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Abstract
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Triplex forming oligonucleotides (TFOs) modified with C5-alkynyl functionalized LNA (locked nucleic acid) monomers display extraordinary thermal affinity toward double stranded DNA targets, excellent discrimination of Hoogsteen-mismatched targets, and high stability against 3′-exonucleases.

Supplementary Info

Synthetic outline of phosphoramidites, protocols forsynthesis/purification of TFOs, determination of T_m- and k_on-values, and exonuclease studies; MALDI-TOF-MS data ofTFOs; thermal denaturation profiles; PAGE experiments; absorptiondecay profiles; discussion regarding parallel duplexformation
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Articles By

Sujay P. Sau
Pawan Kumar
Brooke A. Anderson
Michael E. Østergaard
Lee Deobald
Andrzej Paszczynski
Pawan K. Sharma
Patrick J. Hrdlicka

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Chemical Communications

Optimized DNA-targeting using triplex forming C5-alkynyl functionalized LNA

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