Glutathione-bound gold nanoclusters for selective-binding and detection of glutathione S-transferase-fusion proteins from cell lysates

Cheng-Tai Chen; Wei-Jen Chen; Chao-Zong Liu; Ling-Ya Chang; Yu-Chie Chen

doi:10.1039/B916919A

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Chemical Communications

Issue 48, 2009

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Glutathione-bound gold nanoclusters for selective-binding and detection of glutathione S-transferase-fusion proteins from cell lysates

Cheng-Tai Chen , Wei-Jen Chen , Chao-Zong Liu , Ling-Ya Chang and Yu-Chie Chen

Chem. Commun., 2009, 7515-7517

DOI: 10.1039/B916919A
Received 17 Aug 2009, Accepted 19 Oct 2009
First published on the web 03 Nov 2009

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Abstract
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A straightforward method for the rapid detection of the presence of glutathione S-transferase (GST)-tagged proteins from sample solutions using glutathione (GSH)-bound gold nanoclusters (Au@GSH NCs) with luminescence properties as the detection probes by simple observation with the naked eye was proposed in this study.

Supplementary Info

Characterization data of the Au NCs and identifications ofthe proteins trapped by the NCs are provided
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Cheng-Tai Chen
Wei-Jen Chen
Chao-Zong Liu
Ling-Ya Chang
Yu-Chie Chen

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Chemical Communications

Glutathione-bound gold nanoclusters for selective-binding and detection of glutathione S-transferase-fusion proteins from cell lysates

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