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Issue 18, 2009
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Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Aélig Robin,a  Gareth A. Roberts,b  Johannes Kisch,a  Federico Sabbadin,c  Gideon Grogan,d  Neil Bruce,c  Nicholas J. Turnera  and  Sabine L. Flitsch*a 
Author affiliations

* Corresponding authors

a School of Chemistry and Manchester Interdisciplinary Biocentre (MIB), University of Manchester, 131 Princess Street, Manchester, UK
E-mail: sabine.flitsch@manchester.ac.uk
Fax: +44 (0)161 2751311
Tel: +44 (0)161 3065172

b School of Chemistry, University of Edinburgh, The King’s Buildings, West Mains road, Edinburgh, UK
E-mail: gareth.roberts@ed.ac.uk
Tel: +44 (0)131 650 7779

c Department of Biology, University of York, PO Box 373, York, UK
E-mail: ncb5@york.ac.uk
Tel: +44 (0)1904 328777

d Department of Chemistry, University of York, Heslington, York, UK
E-mail: grogan@ysbl.york.ac.uk
Tel: +44 (0)1904 328256

Abstract

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

Graphical abstract: Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

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Publication details

The article was received on 27 Jan 2009, accepted on 27 Feb 2009 and first published on 24 Mar 2009


Article type: Communication
DOI: 10.1039/B901716J
Citation: Chem. Commun., 2009,0, 2478-2480
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    Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

    A. Robin, G. A. Roberts, J. Kisch, F. Sabbadin, G. Grogan, N. Bruce, N. J. Turner and S. L. Flitsch, Chem. Commun., 2009, 0, 2478
    DOI: 10.1039/B901716J

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