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Issue 9, 2009
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Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

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Abstract

We demonstrate that thiouredopyrene-3,6,8-trisulfonate (TUPS), a photoactivatable reagent, can rapidly inject electrons into complex redox enzymes, enabling studies of the kinetics of internal electron that are not accessible using conventional rapid mixing, stopped-flow methods.

Graphical abstract: Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

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Publication details

The article was received on 14 Nov 2008, accepted on 15 Dec 2008 and first published on 12 Jan 2009


Article type: Communication
DOI: 10.1039/B820386E
Citation: Chem. Commun., 2009,0, 1124-1126
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    Internal electron transfer in multi-site redox enzymes is accessed by laser excitation of thiouredopyrene-3,6,8-trisulfonate (TUPS)

    D. J. Heyes, A. Quinn, P. M. Cullis, M. Lee, A. W. Munro and N. S. Scrutton, Chem. Commun., 2009, 0, 1124
    DOI: 10.1039/B820386E

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