Detection of single-nucleotide mismatches using scanning electrochemical microscopy

Piotr Michal Diakowski; Heinz-Bernhard Kraatz

doi:10.1039/B819876D

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Chemical Communications

Issue 10, 2009

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Detection of single-nucleotide mismatches using scanning electrochemical microscopy

Piotr Michal Diakowski and Heinz-Bernhard Kraatz

Chem. Commun., 2009, 1189-1191

DOI: 10.1039/B819876D
Received 06 Nov 2008, Accepted 03 Dec 2008
First published on the web 05 Jan 2009

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Probing ds-DNA films in the presence of Zn²⁺ ions by scanning electrochemical microscopy (SECM) allows the unequivocal detection of a single-nucleotide mismatch and provides information about its position within the duplex.

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Experimental methods and conditions, microarray spottingprocedure, and estimation of diffusion coefficient andtip–substrate separation
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Chemical Communications

Detection of single-nucleotide mismatches using scanning electrochemical microscopy

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