In vitro        biosynthesis of bacterial peptidoglycan using D-Cys-containing precursors: fluorescent detection of transglycosylation and transpeptidation

Virginie Vinatier; Carl B. Blakey; Darren Braddick; Benjamin R. G. Johnson; Stephen D. Evans; Timothy D. H. Bugg

doi:10.1039/B819869A

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In vitrobiosynthesis of bacterial peptidoglycan using d-Cys-containing precursors: fluorescent detection of transglycosylation and transpeptidation†‡

Virginie Vinatier,^a Carl B. Blakey,^a Darren Braddick,^a Benjamin R. G. Johnson,^b Stephen D. Evans^b and Timothy D. H. Bugg*^a

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Warwick, Coventry, UK
E-mail: T.D.Bugg@warwick.ac.uk
Fax: +44 (0)2476-524112
Tel: +44 (0)2476-573018

^b School of Physics and Astronomy, University of Leeds, Leeds, UK
E-mail: s.d.evans@leeds.ac.uk

Abstract

Peptidoglycan precursors containing D-Cys at position 4 were polymerised to form a synthetic peptidoglycan layer, which could be fluorescently labelled, providing a new method to monitor peptidoglycan transglycosylation and transpeptidation.

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Supplementary files

ES-MS data for fluorescently-labelled peptidoglycan fragments from digestion with lysozyme and MurNAc-L-Ala amidase, and procedures for surface plasmon resonance studies PDF (570K)

Article information

DOI: https://doi.org/10.1039/B819869A
Article type: Communication
Submitted: 06 Nov 2008
Accepted: 02 Jun 2009
First published: 10 Jun 2009

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Chem. Commun., 2009, 4037-4039

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In vitro biosynthesis of bacterial peptidoglycan using D-Cys-containing precursors: fluorescent detection of transglycosylation and transpeptidation

V. Vinatier, C. B. Blakey, D. Braddick, B. R. G. Johnson, S. D. Evans and T. D. H. Bugg, Chem. Commun., 2009, 4037 DOI: 10.1039/B819869A

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