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Issue 3, 2009
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Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains

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Abstract

The layer-by-layer adsorption technique based on the consecutive deposition of oppositely charged species is suitable for the preparation of protein multilayers with fully electro-active protein molecules. The methodology was established with cytochrome c and the polyelectrolyte sulfonated polyaniline (PASA). The technique is also useful for the construction of bi-protein architectures confining proteinprotein communication to an electrode. Following natural examples of protein complexes with defined signal transfer, cytochrome c was arranged with enzymes such as xanthine oxidase, bilirubin oxidase, laccase, and sulfite oxidase in self-assembled multilayer architectures. Thus, biomimetic signal chains from the enzyme substrate via the enzyme and cytochrome c towards the electrode can be established. Communication between proteins immobilised in multiple layers on the electrode can be achieved by in situ generation of small shuttle molecules or more advantageously by direct interprotein electron transfer. This allows the construction of new sensing electrodes, the properties of which can be tuned by the number of deposited protein layers. The mechanism of electron transfer within such protein assemblies on gold electrodes will be discussed.

Graphical abstract: Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains

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Publication details

The article was received on 05 Aug 2008, accepted on 25 Sep 2008 and first published on 12 Nov 2008


Article type: Feature Article
DOI: 10.1039/B813559B
Citation: Chem. Commun., 2009, 274-283
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    Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains

    F. Lisdat, R. Dronov, H. Möhwald, F. W. Scheller and D. G. Kurth, Chem. Commun., 2009, 274
    DOI: 10.1039/B813559B

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