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Issue 10, 2009
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Boronate affinity monolith for highly selective enrichment of glycopeptides and glycoproteins

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Abstract

A novel boronate affinity monolith, poly(3-acrylamidophenylboronic acid-co-ethylene dimethacrylate) (AAPBA-co-EDMA), was prepared in 530 µm capillaries by a one-step in situpolymerization procedure using a pre-polymerization mixture consisting of functional monomer 3-acrylamidophenylboronic acid, cross-linker ethylene dimethacrylate, porogenic solvent methanol with added poly(ethylene glycol) 20 000 (PEG 20 000) and initiator azobisisobutyronitrile (AIBN). The preparation of the monolith was optimized by investigating the ratio of functional monomer to cross-linker and the effect of poly(ethylene glycol) molecular weight. The resulting boronate monolith was used as a sorbent for polymer monolith microextraction (PMME). Using nucleosides as the testing analyte, the extraction performance of this boronate monolith towards glycol-containing compounds was examined. Finally, the boronate monolith was applied for selective enrichment of glycopeptides and glycoproteins.

Graphical abstract: Boronate affinity monolith for highly selective enrichment of glycopeptides and glycoproteins

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Publication details

The article was received on 15 May 2009, accepted on 10 Aug 2009 and first published on 28 Aug 2009


Article type: Paper
DOI: 10.1039/B909581K
Citation: Analyst, 2009,134, 2158-2164
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    Boronate affinity monolith for highly selective enrichment of glycopeptides and glycoproteins

    M. Chen, Y. Lu, Q. Ma, L. Guo and Y. Feng, Analyst, 2009, 134, 2158
    DOI: 10.1039/B909581K

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