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Issue 6, 2009
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Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy

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Abstract

In this study we obtained Fourier transform infrared (FTIR) spectra of fixed prostate cell lines of differing types as well as the primary epithelial cells from benign prostatic hyperplasia (BPH). Results showed that by using multivariate chemometric analysis it was possible to discriminate and classify these cell lines, which gave rise to sensitivity and specificity values of >94% and >98%, respectively. Following on from these results the possible influences of different factors on the discrimination and classification of the prostate cell lines were examined. Firstly, the effect of using different growth media during cell culturing was investigated, with results indicating that this did not influence chemometric discrimination. Secondly, differences in the nucleus-to-cytoplasm (N/C) ratio were examined, and it was concluded that this factor was not the main reason for the discrimination and classification of the prostate cancer (CaP) cell lines. In conclusion, given the fact that neither growth media nor N/C ratio could totally explain the classification it is likely that actual biochemical differences between the cell lines is the major contributing factor.

Graphical abstract: Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy

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Publication details

The article was received on 16 Feb 2009, accepted on 07 Apr 2009 and first published on 17 Apr 2009


Article type: Paper
DOI: 10.1039/B903249E
Citation: Analyst, 2009,134, 1083-1091
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    Factors influencing the discrimination and classification of prostate cancer cell lines by FTIR microspectroscopy

    T. J. Harvey, E. Gazi, A. Henderson, R. D. Snook, N. W. Clarke, M. Brown and P. Gardner, Analyst, 2009, 134, 1083
    DOI: 10.1039/B903249E

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