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Issue 5, 2009
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In vivo recognition of Bacillus subtilis by desorption electrospray ionization mass spectrometry (DESI-MS)

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Abstract

Desorption electrospray ionization mass spectrometry (DESI-MS) of culture of the bacterium Bacillus subtilis as a biofilm growing on agar nutrient gives simple, high quality mass spectra dominated in both the positive and negative ion modes by signals due to the cyclic lipopeptide, Surfactin(C15). This in vivo experiment, performed by direct analysis of untreated microorganism samples under ambient conditions, allows rapid identification of this microorganism and the antibiotics that it produces. The result is suggestive of the capabilities of DESI-MS for in vivo microorganism characterization in general and for monitoring fermentation processes for the production of antibiotics and other biochemicals.

Graphical abstract: In vivo recognition of Bacillus subtilis by desorption electrospray ionization mass spectrometry (DESI-MS)

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Publication details

The article was received on 07 Jan 2009, accepted on 08 Mar 2009 and first published on 16 Mar 2009


Article type: Communication
DOI: 10.1039/B900069K
Citation: Analyst, 2009,134, 838-841
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    In vivo recognition of Bacillus subtilis by desorption electrospray ionization mass spectrometry (DESI-MS)

    Y. Song, N. Talaty, K. Datsenko, B. L. Wanner and R. G. Cooks, Analyst, 2009, 134, 838
    DOI: 10.1039/B900069K

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