Jump to main content
Jump to site search

Issue 6, 2009
Previous Article Next Article

Characterization of Barrett esophagus and esophageal adenocarcinoma by Fourier-transform infrared microscopy

Author affiliations

Abstract

The objective of this exploratory study was to evaluate the feasibility of using Fourier-Transform Infrared (FTIR) spectromicroscopy to characterize formalin-fixed, paraffin-embedded human esophageal tissues. Matched histologically normal esophageal squamous epithelium (NS), premalignant Barrett esophagus (BE), and primary esophageal adenocarcinoma (EADC) tissues, each defined according to strict clinicopathologic criteria, were obtained from patients who underwent esophageal resection. Using confocal IR microscopy, measurements in the mid-IR spectral region were carried out in transflection configuration, scanning regions of interest in 15 µm steps. A multidimensional dataset reporting the spectroscopic properties at each sampled point were analyzed by performing a hierarchical cluster analysis on the second derivative of spectral traces. Normal esophageal epithelia were characterized by a few well defined regions, mostly of large size (tens of contiguous pixels), which correlated with tissue histology, specifically the basal cell layer. BE tissues had characteristic regions localized to gland crypts, ranging in size from one pixel to a few tens of pixels, which displayed IR spectra with defined absorption features characteristic of glycoproteins. The incorporation of synchrotron light to improve the resolution of individual cells in BE tissues has demonstrated that these glycoproteins are associated with goblet cells, the characteristic cell type defining BE. Whereas the highly fragmented regions identified in EADC likely reflect tumor heterogeneity, FTIR mapping would appear to be a potentially useful technique to identify premalignant BE tissues. The technical feasibility of using FTIR to characterize formalin-fixed, paraffin-embedded human esophageal tissues demonstrates the potential of this technique to study archival human BE tissue specimens via automated screening techniques.

Graphical abstract: Characterization of Barrett esophagus and esophageal adenocarcinoma by Fourier-transform infrared microscopy

Back to tab navigation

Publication details

The article was received on 07 Jan 2009, accepted on 21 Jan 2009 and first published on 11 Feb 2009


Article type: Paper
DOI: 10.1039/B823071D
Citation: Analyst, 2009,134, 1240-1246
  •   Request permissions

    Characterization of Barrett esophagus and esophageal adenocarcinoma by Fourier-transform infrared microscopy

    L. Quaroni and A. G. Casson, Analyst, 2009, 134, 1240
    DOI: 10.1039/B823071D

Search articles by author

Spotlight

Advertisements