Phenotypic heterogeneity within microbial populations at the single-cell level investigated by confocal Raman microspectroscopy

Antje Hermelink; Angelika Brauer; Peter Lasch; Dieter Naumann

doi:10.1039/B822574E

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Analyst

Issue 6, 2009

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Phenotypic heterogeneity within microbial populations at the single-cell level investigated by confocal Raman microspectroscopy

Antje Hermelink , Angelika Brauer , Peter Lasch and Dieter Naumann

Analyst, 2009, 134, 1149-1153

DOI: 10.1039/B822574E
Received 16 Dec 2008, Accepted 12 Mar 2009
First published on the web 25 Mar 2009
This article is part of the collection: Optical Diagnosis

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Single cells in genetically homogeneous microbial cultures exhibit marked phenotypic heterogeneity that is considered to bolster the fitness of the whole population. Heterogeneity on the single-cell level is typically masked in conventional studies of microbial populations, which rely on data averaged across thousands or millions of cells in a sample. Here we introduce confocal Raman microspectroscopy as a method for investigating and illustrating the spatial heterogeneity of microbial cell populations. By the use of three different test organisms as model systems, we show pronounced cellular heterogeneity even in colonies cultivated under laboratory conditions.

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Phenotypic heterogeneity within microbial populations at the single-cell level investigated by confocal Raman microspectroscopy

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