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Issue 7, 2009
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Sensitive and selective detection of cysteine using gold nanoparticles as colorimetric probes

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Abstract

We report herein the development of a highly sensitive and selective colorimetric detection method for cysteine using gold nanoparticles probes. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the self-assembly of cysteine on gold nanoparticles, and the interaction of a 2:1 cysteine/Cu2+ complex. In the presence of Cu2+, cysteine could rapidly induce the aggregation of gold nanoparticles, thereby resulting in red-to-blue (or purple) color change. The concentration of cysteine can be determined by monitoring with the naked eye or a UV-vis spectrometer. The present limit of detection for cysteine is 10 nM. This method exhibits excellent selectivity for cysteine over other α-amino acids, glutathione, thioglycolic acid and mercaptoethyl alcohol.

Graphical abstract: Sensitive and selective detection of cysteine using gold nanoparticles as colorimetric probes

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Publication details

The article was received on 06 Nov 2008, accepted on 23 Mar 2009 and first published on 02 Apr 2009


Article type: Paper
DOI: 10.1039/B819842J
Citation: Analyst, 2009,134, 1361-1365
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    Sensitive and selective detection of cysteine using gold nanoparticles as colorimetric probes

    L. Li and B. Li, Analyst, 2009, 134, 1361
    DOI: 10.1039/B819842J

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