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Issue 4, 2009
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Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor

Dian-Bing Wang,ac  Li-Jun Bi,b  Zhi-Ping Zhang,a  Yuan-Yuan Chen,b  Rui-Fu Yang,d  Hong-Ping Wei,a  Ya-Feng Zhoua  and  Xian-En Zhang*a 
Author affiliations

* Corresponding authors

a State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
E-mail: x.zhang@wh.iov.cn
Fax: +86 27 87199492
Tel: +86 10 58881508

b State Key Laboratory of Macromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China

c State Key Laboratory of Agromicrobiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China

d State Key Laboratory of Pathogen and Biosecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China

Abstract

This study demonstrates the first use of surface plasmon resonance (SPR) technology for the rapid, sensitive and label-free detection of whole B. anthracis spores. The approach involves the use of an SPR biosensor (Biacore® 3000), and a monoclonal antibody which was raised against the B. anthracis spore (mAb 8G3). By means of subtractive inhibition assays, whole B. anthracis spores with concentrations as low as 104 colony-forming units (CFU) ml−1 can be detected within 40 min, and other related Bacillus spores, even in high concentrations, can be differentiated from B. anthracis spores.

Graphical abstract: Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor

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Publication details

The article was received on 29 Jul 2008, accepted on 02 Dec 2008 and first published on 22 Jan 2009


Article type: Paper
DOI: 10.1039/B813038H
Citation: Analyst, 2009,134, 738-742
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    Label-free detection of B. anthracis spores using a surface plasmon resonance biosensor

    D. Wang, L. Bi, Z. Zhang, Y. Chen, R. Yang, H. Wei, Y. Zhou and X. Zhang, Analyst, 2009, 134, 738
    DOI: 10.1039/B813038H

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