Issue 6, 2008
From the journal:

Soft Matter

Direct visualization of phase transition dynamics in binary supported phospholipid bilayers using imaging ellipsometry†

Alan W. Szmodis,ab   Craig D. Blanchette,c   Andrey A. Levchenko,d   Alexandra Navrotsky,d   Marjorie L. Longo,bd   Christine A. Ormee  and  Atul N. Parikh*ab  

* Corresponding authors

a Dept. of Applied Science, University of California, Davis, CA
E-mail: anparikh@ucdavis.edu
Fax: +1 (530)752-2444
Tel: +1 (530)754-7055

b Biophysics Graduate Group, University of California, Davis, CA, USA

c Biophysical and Interfacial Science Group, Chemistry and Material Science, Lawrence Livermore National Laboratory, Livermore, CA, USA

d Chemical Engineering and Materials Science Department, University of California, Davis, CA, USA

e Biosciences and Biotechnology Division, Lawrence Livermore National Laboratory, Livermore, CA, USA

Abstract

Via imaging ellipsometry, we study the phase transition dynamics induced by selective gelation of one component in a binary supported phopholipid bilayer. We find the modulation of two attendant morphological features: emergence of extended defect chains due to a net change in the molecular areas and fractal-like domains suggesting weak line tension. A time-lapse analysis of the ellipsometric images reveals the cluster size of 4–20 molecules undergoing gelation indicating weak cooperativity. These results demonstrate the use of ellipsometry for in situ, label-free, non-contact, and large-area imaging of dynamics in interfacial films.

Graphical abstract: Direct visualization of phase transition dynamics in binary supported phospholipid bilayers using imaging ellipsometry

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Article information

DOI
https://doi.org/10.1039/B801390J
Article type
Communication
Submitted
28 Jan 2008
Accepted
14 Apr 2008
First published
28 Apr 2008

Soft Matter, 2008,4, 1161-1164

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Direct visualization of phase transition dynamics in binary supported phospholipid bilayers using imaging ellipsometry

A. W. Szmodis, C. D. Blanchette, A. A. Levchenko, A. Navrotsky, M. L. Longo, C. A. Orme and A. N. Parikh, Soft Matter, 2008, 4, 1161 DOI: 10.1039/B801390J

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