Peptide- and protein-mediated assembly of heparinized hydrogels

Polymeric hydrogels have demonstrated significant promise in biomedical applications such as drug delivery and tissue engineering. A continued direction in hydrogel development includes the engineering of the biological responsiveness of these materials, via the inclusion of cell-binding domains and enzyme-sensitive domains. Ligand–receptor interactions offer additional opportunities in the design of responsive hydrogels, and strategies employing protein–polysaccharide interactions as a target may have unique relevance to materials intended to mimic the extracellular matrix (ECM). Accordingly, we have developed approaches for producing hydrogels via noncovalent interactions between heparin and heparin-binding peptides/proteins, and have demonstrated that such matrices are capable of both passive and receptor-mediated growth factor delivery. Further modification of these materials via the integration of these noncovalent strategies with chemical crosslinking methods will expand the range of their potential use and is under exploration. The combination of these approaches offers broad opportunities for the production of responsive matrices for biomedical applications.