Jump to main content
Jump to site search

Issue 11, 2008
Previous Article Next Article

Characterization of new fluorescent labels for ultra-high resolution microscopy

Author affiliations

Abstract

Photo-induced switching of dyes into dark, long-lived states, such as a triplet state, has recently gained increasing interest, as a means to achieve ultra-high optical resolution. Additionally, these long lived states are often highly environment-sensitive and their photodynamics can thus offer additional independent fluorescence-based information. However, although providing a useful mechanism for photo-induced switching, the triplet state often appears as a precursor state for photobleaching, which potentially can limit its usefulness. In this work, a set of rhodamine and pyronin dyes, modified by substitution of heavy atoms and nitrogen within or close to the central xanthene unit of the dyes, were investigated with respect to their triplet state dynamics and photostabilities, under conditions relevant for ultra-high resolution microscopy. Out of the dyes investigated, in particular the rhodamine and pyronin dyes with a sulfur atom replacing the central oxygen atom in the xanthene unit were found to meet the requirements for ultra-high resolution microscopy, combining a prominent triplet state yield with reasonable photostability.

Graphical abstract: Characterization of new fluorescent labels for ultra-high resolution microscopy

Back to tab navigation

Publication details

The article was received on 17 Jun 2008, accepted on 11 Sep 2008 and first published on 30 Sep 2008


Article type: Paper
DOI: 10.1039/B810991P
Citation: Photochem. Photobiol. Sci., 2008,7, 1378-1385
  •   Request permissions

    Characterization of new fluorescent labels for ultra-high resolution microscopy

    A. Chmyrov, J. Arden-Jacob, A. Zilles, K. Drexhage and J. Widengren, Photochem. Photobiol. Sci., 2008, 7, 1378
    DOI: 10.1039/B810991P

Search articles by author

Spotlight

Advertisements