Time-resolved methods in biophysics. 7. Photon counting vs. analog time-resolved singlet oxygen phosphorescence detection

Ana Jiménez-Banzo; Xavier Ragàs; Peter Kapusta; Santi Nonell

doi:10.1039/B804333G

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Photochemical & Photobiological Sciences

Issue 9, 2008

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Time-resolved methods in biophysics. 7. Photon counting vs. analog time-resolved singlet oxygen phosphorescence detection

Ana Jiménez-Banzo , Xavier Ragàs , Peter Kapusta and Santi Nonell

Photochem. Photobiol. Sci., 2008, 7, 1003-1010

DOI: 10.1039/B804333G
Received 13 Mar 2008, Accepted 09 May 2008
First published on the web 25 Jun 2008
This article is part of the collection: Time-resolved methods in biophysics series

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Abstract
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Two recent advances in optoelectronics, namely novel near-IR sensitive photomultipliers and inexpensive yet powerful diode-pumped solid-state lasers working at kHz repetition rate, enable the time-resolved detection of singlet oxygen (O₂(a¹Δ_g)) phosphorescence in photon counting mode, thereby boosting the time-resolution, sensitivity, and dynamic range of this well-established detection technique. Principles underlying this novel approach and selected examples of applications are provided in this perspective, which illustrate the advantages over the conventional analog detection mode.

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Photochemical & Photobiological Sciences

Time-resolved methods in biophysics. 7. Photon counting vs. analog time-resolved singlet oxygen phosphorescence detection

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