Issue 20, 2008

Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA

Abstract

dATP derivatives bearing Br, Me or Ph groups in position 8 were prepared and tested as substrates for DNA polymerases to show that 8-Br-dATP and 8-Me-dATP were efficiently incorporated, while 8-Ph-dATP was a poor substrate due to its bulky Ph group.

Graphical abstract: Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA

Supplementary files

Article information

Article type
Communication
Submitted
11 Jul 2008
Accepted
29 Aug 2008
First published
17 Sep 2008

Org. Biomol. Chem., 2008,6, 3657-3660

Synthesis of 8-bromo-, 8-methyl- and 8-phenyl-dATP and their polymerase incorporation into DNA

H. Cahová, R. Pohl, L. Bednárová, K. Nováková, J. Cvačka and M. Hocek, Org. Biomol. Chem., 2008, 6, 3657 DOI: 10.1039/B811935J

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