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Issue 4, 2008
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Alloxazine as a ligand for selective binding to adenine opposite AP sites in DNA duplexes and analysis of single-nucleotide polymorphisms

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Abstract

Alloxazine can bind to adenine selectively over other nucleobases opposite an abasic site in DNA duplexes (5′-TCC AG[X with combining low line] GCA AC-3′/3′-AGG TC[N with combining low line] CGT TG-5′, [X with combining low line] = AP site, [N with combining low line] = A, T, C, G) with a dissociation constant of 0.82 µM (pH 7.0, I = 0.11 M, at 5 °C), and it is applicable to SNPs typing of PCR amplification products based on the binding-induced fluorescence response.

Graphical abstract: Alloxazine as a ligand for selective binding to adenine opposite AP sites in DNA duplexes and analysis of single-nucleotide polymorphisms

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Publication details

The article was received on 02 Jan 2008, accepted on 07 Jan 2008 and first published on 18 Jan 2008


Article type: Communication
DOI: 10.1039/B719786A
Citation: Org. Biomol. Chem., 2008,6, 670-673
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    Alloxazine as a ligand for selective binding to adenine opposite AP sites in DNA duplexes and analysis of single-nucleotide polymorphisms

    B. Rajendar, S. Nishizawa and N. Teramae, Org. Biomol. Chem., 2008, 6, 670
    DOI: 10.1039/B719786A

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